Transgenic poplar(Populus alba×P.glandulosa cv‘84k’) plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar’s toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.

目的 研究Esteya vermicola真菌适应的pH范围，并探讨松树成分对其生长的影响，以期为E. vermicola菌在松树体内的定植和林业重大病害松材线虫野外的生物防治提供理论支撑。 方法 选取E. vermicolaCBS115803（EV115803）和E. vermicolaCNU120806（EV120806）为试验对象，测试EV真菌适应的pH范围，并以35 a黑松不同器官组织配制培养基，探究EV菌的生长情况，观测各菌株生长速率、菌体量和产孢量。 结果 EV菌适应的pH值范围为5～13，菌体量在酸性条件下产生更多的气生菌丝。PDA中加入松树成分对EV菌有一定的刺激生长作用。在以松针、松树皮、松枝条为唯一营养来源时，EV菌生长茂盛。以木质部锯末为单一营养源时，EV菌生长缓慢。 结论 EV菌适应的pH值范围为5～13，EV菌耐碱性极强，且在加有松树各部位成分的培养基上生长，菌株EV120806对松树培养基的适应性更强，未来有望将EV菌定殖在松树中做为共生菌，摄食入侵的松材线虫，从而使松树具有内源的防控因子防控松材线虫。

用不同浓度的HaNPV病毒感染3龄棉铃虫幼虫并分别收集不同处理的存活试虫进行室内传代饲养。结果表明,病毒对寄主昆虫不仅具有直接致死作用,而且对蛹质量和化蛹均有明显的影响,尤其是对亲代和子一代的寄主昆虫。同时,通过PCR技术,从子一代的幼虫和蛹中成功检测到该病毒,从而在分子水平上证明了病毒能够经过亲代传递到子代的种群中。这对病毒的高效利用和害虫的持续控制具有重要的理论和实践意义。

This paper reported the biological activities of five synthetic philanthotoxin analogs.Trials under laboratory condition indicated a certain level of activity on poisoning and inhibiting emergence rate.The LC50 of compound 1 on Ectropis obliqua was 8.987×103 mg·L-1.At the 8×103 mg·L-1 dosage of compound 1,the adjustment rate of pupation and emergence of E.obliqua was 52.44% and 89.81% respectively.The compound 2 had a certain effect on the larval and pupal weight.

根据茶尺蠖核型多角体病毒(EoNPV)多角体蛋白基因建立了PCR检测方法,在茶尺蠖子代卵和蛹内检测到EoNPV存在,灵敏度达到1 fg,此方法可用于田间施用EoNPV后茶尺蠖种群带毒检测。PCR方法由于操作简单、检测灵敏度高、特异性好、重复性好等优点,值得应用。利用EoNPV感染茶尺蠖2龄幼虫,幼虫对EoNPV的敏感性很高,测得LC50为2. 2 ×104 P IB·mL-l , EoNPV感染浓度越高,则幼虫死亡越快,幼虫死亡时间与感染浓度呈正相关。

The combinations of Hyphantria cunea NPV (HcNPV) and three different fluorescent brighteners BA,VBL, CBS-X were added to the artificial diet and the influence of synergists in concentration 25 ℃ and 14L /10D( h). The results showed that the fluorescent brightenersBA and VBL had higher insecticidal activity, CBS-X had notsignificant synergistic effect.

Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.

Primers were designed according to sequence of polyhedron envelope protein gene(pep) of BusuNPV, then the polymerase chain reaction(PCR) technology was used to detect Buzura suppressaria nucleopolyhedrovirus DNA sequences from viral polyhedral inclusion bodies(PIB), and the DNA sequences was detected from the egg and offspring.The level of sensitivity by the technology was as low as 1 fg·mL-1 DNA.Morphology and biological activity studies of BusuNPV showed that,the BusuNPV saved by laboratory was single-nucleocapsid nucleopolyhedrovirus (SNPV).An infectivity test of BusuNPV against the second instar larvae of Buzura suppressaria was carried out in out laboratory,and LC50 of BusuNPV was determined as 6.65×104 PIB·mL-1, and LT50 of BusuNPV was 5.69 d.

从不同培养基、不同测定方法、不同菌株、不同孢子浓度4个方面测定内寄生真菌Esteya vermicola孢子对松材线虫的侵染活力，结果表明：该真菌离体分生孢子对松材线虫有侵染活力；PDA固体培养比PDB液体培养所得孢子能更有效的粘附和杀死松材线虫；在WA平板上，采用浸渍法测定孢子对线虫的侵染活力比其他处理效果好；在供试的3个菌株（CUN 120806、CBS 10082和CBS 115803）中，CBS 115803菌株对松材线虫具有较高侵染活性；4个不同浓度（1×109、1×108、1×107、1×106个·mL-1）孢子对线虫的作用中，随着孢子浓度降低，孢子对线虫侵染能力也随之降低。

根据舞毒蛾核型多角体病毒(LdMNPV)的蜕皮甾体尿苷二磷酸葡萄糖基转移酶基因(egt)设计一对特异性引物,成功建立了LdMNPV的PCR检测技术体系,其对LdMNPV基因组的灵敏度可达到1 fg·mL-1。应用该技术体系从舞毒蛾带毒幼虫、卵、蛹的基因组中扩增出目的片段,证明了这一技术的可行性。对不同浓度的多角体悬液的扩增结果显示:其检测最低量为5 OBs·mL-1。形态学研究表明:从内蒙舞毒蛾体内分离到的LdMNPV中,少数表面有凹陷的孔洞,病毒粒子从这些孔洞中游离出来,这也阐明了此技术能够从多角体悬液中扩增出目的片段的原因。在将来LdMNPV的检测中,可用虫体进行研磨过滤离心得到的组织液作为模板进行扩增。